Advanced Optical Flow Cytometry: Methods and Disease Diagnoses
Authors: Tuchin - Valery V. (Editor)
ISBN-10: 3527409343
ISBN-13: 9783527409341
Edition: 1
Release: May 16, 2011
Hardcover: 740 pages
List Price $170.00
作者简介
Valery V. Tuchin is Professor of Optics and Biophotonics and a Director of the Research-Educational Institute of Optics and Biophotonics at Saratov State University, Russia, as well as Head of the Laboratory on Laser Diagnostics of Technical and Living Systems, Institute of Precise Mechanics and Control, RAS. He has more than 300 publications to his name, and is a recipient of the Honored Science Worker and SPIE Educator Award. Professor Tuchin is a SPIE Fellow and Vice-President of the Russian Photobiology Society. His research interests include biophotonics, biomedical optics and laser medicine, physics of optical and laser measurements.
目录
Preface.
List of Contributors.
1 Perspectives in Cytometry (Anja Mittag and Attila Tá
rnok).
1.1 Background.
1.2 Basics of Cytometry.
1.3 Cytomics.
1.4 Cytometry – State of the Art.
1.5 Perspectives.
1.6 Conclusion.
References.
2 Novel Concepts and Requirements in Cytometry (Herbert Schneckenburger, Michael Wagner, Petra Weber, and Thomas Bruns).
2.1 Introduction.
2.2 Fluorescence Microscopy.
2.3 Fluorescence Reader Systems.
2.4 Microfluidics Based on Optical Tweezers.
2.5 Conclusion.
Acknowledgment.
References.
3 Optical Imaging of Cells with Gold Nanoparticle Clusters as Light Scattering Contrast Agents: A Finite-Difference Time-Domain Approach to the Modeling of Flow Cytometry Configurations (Stoyan Tanev, Wenbo Sun, James Pond, Valery V. Tuchin, and Vladimir P. Zharov).
3.1 Introduction.
3.2 Fundamentals of the FDTD Method.
3.3 FDTD Simulation Results of Light Scattering Patterns from Single Cells.
3.4 FDTD OPCM Nanobioimaging Simulation Results.
3.5 Conclusion.
Acknowledgment.
References.
4 Optics of White Blood Cells: Optical Models, Simulations, and Experiments (Valeri P. Maltsev, Alfons G. Hoekstra, and Maxim A. Yurkin).
4.1 Introduction.
4.2 Optical Models of White Blood Cells.
4.3 Direct and Inverse Light-Scattering Problems for White Blood Cells.
4.4 Experimental Measurement of Light Scattering by White Blood Cells.
4.5 Conclusion.
Acknowledgments.
References.
5 Optical Properties of Flowing Blood Cells (Martina C. Meinke, Moritz Friebel, and Jü
rgen Helfmann).
5.1 Introduction.
5.2 Blood Physiology.
5.3 Complex Refractive Index of Hemoglobin.
5.4 Light Propagation in Turbid Media.
5.5 Method for the Determination of Optical Properties of Turbid Media.
5.6 Optical Properties of Red Blood Cells.
5.7 Optical Properties of Plasma.
5.8 Optical Properties of Platelets.
5.9 Comparison of Optical Influences Induced by Physiological Blood Parameters.
5.10 Summary.
Acknowledgments.
References.
6 Laser Diffraction by the Erythrocytes and Deformability Measurements (Sergei Yu. Nikitin, Alexander V. Priezzhev, and Andrei E. Lugovtsov).
6.1 Introduction.
6.2 Parameters of the Erythrocytes.
6.3 Parameters of the Ektacytometer.
6.4 Light Scattering by a Large Optically Soft Particle.
6.5 Fraunhofer Diffraction.
6.6 Light Scattering by a Transparent Elliptical Disc.
6.7 Light Scattering by an Elliptical Disc with Arbitrary Coordinates of the Disc Center.
6.8 Light Diffraction by an Ensemble of Particles.
6.9 Light Diffraction by Particles with Random Coordinates.
6.10 Light Scattering by Particles with Regular Coordinates.
6.11 Description of the Experimental Setup.
6.12 Sample Preparation Procedure.
6.13 Examples of Experimental Assessment of Erythrocyte Deformability in Norm and Pathology.
6.14 Conclusion.
References.
7 Characterization of Red Blood Cells’ Rheological and Physiological State Using Optical Flicker Spectroscopy (Vadim L. Kononenko).
7.1 Introduction.
7.2 Cell State-Dependent Mechanical Properties of Red Blood Cells.
7.3 Flicker in Erythrocytes.
7.4 Experimental Techniques for Flicker Measurement in Blood Cells.
7.5 The Measured Quantities in Flicker Spectroscopy and the Cell Parameters Monitored.
7.6 Flicker Spectrum Influence by Factors of Various Nature.
7.7 Membrane Flicker and Erythrocyte Functioning.
7.8 Flicker in Other Cells.
7.9 Conclusions.
References.
8 Digital Holographic Microscopy for Quantitative Live Cell Imaging and Cytometry (Bjö
rn Kemper and J¨
urgen Schnekenburger).
8.1 Introduction, Motivation, and Background.
8.2 Principle of DHM.
8.3 DHM in Cell Analysis.
8.4 Conclusion.
Acknowledgment.
References.
9 Comparison of Immunophenotyping and Rare Cell Detection by Slide-Based Imaging Cytometry and Flow Cytometry (Jó
zsef Bocsi, Anja Mittag, and Attila Tá
rnok).
9.1 Introduction.
9.2 Comparison of Four-Color CD4/CD8 Leukocyte Analysis by SFM and FCM Using Qdot Staining.
9.3 Comparison of Leukocyte Subtyping by Multiparametric Analysis with LSC and FCM.
9.4 Absolute and Relative Tumor Cell Frequency Determinations.
9.5 Analysis of Drug-Induced Apoptosis in Leukocytes by Propidium Iodide.
9.6 Conclusion.
Acknowledgment.
References.
10 Microfluidic Flow Cytometry: Advancements toward Compact, Integrated Systems (Shawn O. Meade, Jessica Godin, Chun-Hao Chen, Sung Hwan Cho, Frank S. Tsai, Wen Qiao, and Yu-Hwa Lo).
10.1 Introduction.
10.2 On-Chip Flow Confinement.
10.3 Optical Detection System.
10.4 On-Chip Sorting.
10.5 Conclusion.
Acknowledgments.
References.
11 Label-Free Cell Classification with Diffraction Imaging Flow Cytometer (Xin-Hua Hu and Jun Q. Lu).
11.1 Introduction.
11.2 Modeling of Scattered Light.
11.3 FDTD Simulation with 3D Cellular Structures.
11.4 Simulation and Measurement of Diffraction Images.
11.5 Summary.
Acknowledgments.
References.
12 An Integrative Approach for Immune Monitoring of Human Health and Disease by Advanced Flow Cytometry Methods (Rabindra Tirouvanziam, Daisy Diaz, Yael Gernez, Julie Laval, Monique Crubezy, and Megha Makam).
12.1 Introduction.
12.2 Optimized Protocols for Advanced Flow Cytometric Analysis of Human Samples.
12.3 Reagents for Advanced Flow Cytometric Analysis of Human Samples.
12.4 Conclusion: The Future of Advanced Flow Cytometry in Human Research.
Acknowledgments.
Abbreviations.
References.
13 Optical Tweezers and Cytometry (Raktim Dasgupta and Pradeep Kumar Gupta).
13.1 Introduction.
13.2 Optical Tweezers: Manipulating Cells with Light.
13.3 Use of Optical Tweezers for the Measurement of Viscoelastic Parameters of Cells.
13.4 Cytometry with Raman Optical Tweezers.
13.5 Cell Sorting.
13.6 Summary.
References.
14 In vivo Image Flow Cytometry (Valery V. Tuchin, Ekaterina I. Galanzha, and Vladimir P. Zharov).
14.1 Introduction.
14.2 State of the Art of Intravital Microscopy.
14.3 In vivo Lymph Flow Cytometry.
14.4 High-Resolution Single-Cell Imaging in Lymphatics.
14.5 In vivo Blood Flow Cytometry.
14.6 Conclusion.
Acknowledgments.
References.
15 Instrumentation for In vivo Flow Cytometry – a Sickle Cell Anemia Case Study (Stephen P. Morgan and IanM. Stockford).
15.1 Introduction.
15.2 Clinical Need.
15.3 Instrumentation.
15.4 Image Processing.
15.5 Modeling.
15.6 Device Design – Sickle Cell Anemia Imaging System.
15.7 Imaging Results – Sickle Cell Anemia Imaging System.
15.8 Discussion and Future Directions.
References.
16 Advances in Fluorescence-Based In vivo Flow Cytometry for Cancer Applications (Cherry Greiner and Irene Georgakoudi).
16.1 Introduction.
16.2 Background: Cancer Metastasis.
16.3 Clinical Relevance: Role of CTCs in Cancer Development and Response to Treatment.
16.4 Current Methods.
16.5 In vivo Flow Cytometry (IVFC).
16.6 Single-Photon IVFC (SPIVFC).
16.7 Multiphoton IVFC (MPIVFC).
16.8 Summary and Future Directions.
Acknowledgments.
References.
17 In vivo Photothermal and Photoacoustic Flow Cytometry (Valery V. Tuchin, Ekaterina I. Galanzha, and Vladimir P. Zharov).
17.1 Introduction.
17.2 Photothermal and Photoacoustic Effects at Single-Cell Level.
17.3 PT Technique.
17.4 Integrated PTFC for In vivo Studies.
17.5 Integrated PAFC for In vivo Studies.
17.6 In vivo Lymph Flow Cytometery.
17.7 In vivo Mapping of Sentinel Lymph Nodes (SLNs).
17.8 Concluding Remarks and Discussion.
Acknowledgments.
References.
18 Optical Instrumentation for the Measurement of Blood Perfusion, Concentration, and Oxygenation in Living Microcirculation (Martin J. Leahy and Jim O’Doherty).
18.1 Introduction.
18.2 Xe Clearance.
18.3 Nailfold Capillaroscopy.
18.4 LDPM/LDPI.
18.5 Laser Speckle Perfusion Imaging (LSPI).
18.6 TiVi.
18.7 Comparison of TiVi, LSPI, and LDPI.
18.8 Pulse Oximetry.
18.9 Conclusions.
Acknowledgments.
References.
19 Blood Flow Cytometry and Cell Aggregation Study with Laser Speckle (Qingming Luo, Jianjun Qiu, and Pengcheng Li).
19.1 Introduction.
19.2 Laser Speckle Contrast Imaging.
19.3 Investigation of Optimum Imaging Conditions with Numerical Simulation.
19.4 Spatio-Temporal Laser Speckle Contrast Analysis.
19.5 Fast Blood Flow Visualization Using GPU.
19.6 Detecting Aggregation of Red Blood Cells or Platelets Using Laser Speckle.
19.7 Conclusion.
Acknowledgments.
References.
20 Modifications of Optical Properties of Blood during Photodynamic Reactions In vitro and In vivo (Alexandre Douplik, Alexander Stratonnikov, Olga Zhernovaya, and Viktor Loshchenov).
20.1 Introduction.
20.2 Description and Brief History of PDT.
20.3 PDT Mechanisms.
20.4 Blood and PDT.
20.5 Properties of Blood, Blood Cells, and Photosensitizers: Before Photodynamic Reaction.
20.6 Photodynamic Reactions in Blood and Blood Cells, Blood Components, and Cells.
20.7 Types of Photodynamic Reactions in Blood: In vitro versus In vivo.
20.8 Blood Sample In vitro as a Model Studying Photodynamic Reaction.
20.9 Monitoring of Oxygen Consumption and Photobleaching in Blood during PDT In vivo.
20.10 Photodynamic Disinfection of Blood.
20.11 Photodynamic Therapy of Blood Cell Cancer.
20.12 Summary.
Acknowledgments.
Glossary.
References.
Index.
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