Confocal Microscopy Methods and Protocols

Confocal Microscopy Methods and Protocols (Methods in Molecular Biology)

Editorial Reviews Review "This book is an essential reference for anyone who is involved in the production of laser scanning confocal (LSCM) images. . . Exceptionally useful information on a great variety of methodologies that can be employed with LSCM is provided. . . In consideration of the vast quantity of useful information on all aspects of LSCM that is presented in this volume, and the fact that it is the first comprehensive book on this topic, it is highly recommended."-Quarterly Review of Biology "..almost every research institute has a confocal microscope, and many are developing much larger multi-user biomedical imaging facilities. The publication of this collection of methods and protocols for confocal microscopy is therefore very timely..The tips and tricks used on the various systems should prove useful to a diverse range of confocal microscope users and would be a beneficial reference volume for any imaging unit and reasonable value for money."-Cell Biology International " This is an excellent source for anyone who wants to explore techniques in confocal microscopy. It is a practical manual with many gems that will help both new and experienced users. The principles are efficiently and accessibly explained and there is a useful chapter on fluorescent probes..there is a wealth of ideas here waiting to be applied to prokaryotic biology and many would be equally useful for simple fluorescence rather than confocal studies. I would certainly recommend it to any lab that uses fluorescence microscopy extensively."-Microbiology Today "..The texts are aimed at the biological user..for the user for whom they are intended, these essays contain a wealth of down-to-earth practical information. The book is well-produced and contains colour illustrations.."-Ultramicroscopy "..excellent information as an introductory basis for confocal microscopy."-Cellular and Molecular Biology From the Back Cover In Confocal Microscopy Methods and Protocols, Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. Centered in the many biological applications of the confocal microscope, the book makes possible the successful imaging of both fixed and living specimens using primarily the laser scanning confocal microscope. The powerful hands-on methods collected in Confocal Microscopy Methods and Protocols will help even the novice to produce first-class cover-quality confocal images.
Book Description

Paperback: 446 pages
Publisher: Humana Press; 1st edition (January 15, 1999)
Language: English
ISBN-10: 0896035263
ISBN-13: 9780896035263
Stephen W. Paddock(Editor)